Exine dehiscing induces rape microspore polarity, which results in different daughter cell fate and fixes the apical–basal axis of the embryo
نویسندگان
چکیده
The roles of cell polarity and the first asymmetric cell division during early embryogenesis in apical-basal cell fate determination remain unclear. Previously, a novel Brassica napus microspore embryogenesis system was established, by which rape exine-dehisced microspores were induced by physical stress. Unlike traditional microspore culture, cell polarity and subsequent asymmetric division appeared in the exine-dehisced microspore, which finally developed into a typical embryo with a suspensor. Further studies indicated that polarity is critical for apical-basal cell fate determination and suspensor formation. However, the pattern of the first division was not only determined by cell polarity but was also regulated by the position of the ruptured exine. The first division could be equal or unequal, with its orientation essentially perpendicular to the polar axis. In both types of cell division, the two daughter cells could have different cell fates and give rise to an embryo with a suspensor, similar to zygotic apical-basal cell differentiation. The alignment of the two daughter cells is consistent with the orientation of the apical-basal axis of future embryonic development. Thus, the results revealed that exine dehiscing induces rape microspore polarization, and this polarity results in a different cell fate and fixes the apical-basal axis of embryogenesis, but is uncoupled from cell asymmetric division. The present study demonstrated the relationships among cell polarity, asymmetric cell division, and cell fate determination in early embryogenesis.
منابع مشابه
Plasticity in Cell Division Patterns and Auxin Transport Dependency during in Vitro Embryogenesis in Brassica napus.
In Arabidopsis thaliana, zygotic embryo divisions are highly regular, but it is not clear how embryo patterning is established in species or culture systems with irregular cell divisions. We investigated this using the Brassica napus microspore embryogenesis system, where the male gametophyte is reprogrammed in vitro to form haploid embryos in the absence of exogenous growth regulators. Microsp...
متن کاملTranscriptional activation of Arabidopsis axis patterning genes WOX8/9 links zygote polarity to embryo development.
In most flowering plants, the apical-basal body axis is established by an asymmetric division of the polarized zygote. In Arabidopsis, early embryo patterning is regulated by WOX homeobox genes, which are coexpressed in the zygote but become restricted to apical (WOX2) and basal (WOX8/9) cells. How the asymmetry of zygote division is regulated and connected to the daughter cell fates is largely...
متن کاملThe novel peptide HEADY specifies apical fate in a simple radially symmetric metazoan.
One of the first steps in animal development is axis formation, during which an uneven distribution of signals and/or transcription factors results in the establishment of polarity in the embryo. Hydra, one of the simplest metazoan animals, shows characteristics of a permanent embryo. Even adult polyps have a striking capacity to regenerate, suggesting that molecular mechanisms underlying de no...
متن کاملControl of spindle orientation in Drosophila by the Par-3-related PDZ-domain protein Bazooka
BACKGROUND The orientation of the mitotic spindle influences the asymmetric distribution of cytoplasmic determinants and the positioning of the sibling cell, and therefore has important influences on cell-fate determination and patterning of the embryo. Both the establishment of an axis of polarity and the adjustment of this axis with respect to the coordinates of the embryo have to be controll...
متن کاملThe Arabidopsis thaliana gametophytic mutation gemini pollen1 disrupts microspore polarity, division asymmetry and pollen cell fate.
Pollen development and male gametogenesis are critically dependent upon cell polarization leading to a highly asymmetric cell division termed pollen mitosis I. A mutational approach was adopted in Arabidopsis thaliana to identify genes involved these processes. Four independent gemini pollen mutants were isolated which produce divided or twin-celled pollen. The gemini pollen1 mutant was charact...
متن کامل